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. 2024 Nov 26;8(2):e202402823. doi: 10.26508/lsa.202402823

Figure S10. ADD3 does not affect Onda-11 GSC invasiveness.

Figure S10.

Invasive capacity of Onda-11 GSC is measured through the neurosphere Matrigel invasion assay at days 0–7. (A) Representative brightfield images of Onda-11 GSC (control, left; ADD3 OE, right) neurospheres at day 3 post-Matrigel addition (top panels) and day 7 post-Matrigel addition (bottom panels). Scale bars: 200 μm. (B) Invasive index at days 0–7 in Onda-11 GSC neurospheres (control, gray; ADD3 OE, green) measured as a ratio between the total area of the neurosphere and the area of its core. Data are the mean of five independent experiments. The total number of neurospheres at the first time point is as follows: 22 control and 22 ADD3 OE. Error bars, SEM.