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. 2024 Nov 13;11:1493650. doi: 10.3389/fvets.2024.1493650

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Copyright © 2024 He, Xie, Yuan, Ouyang, Dong, Ling, Zeng, Fang, Song, Wang, Ding, Zhao, Fan, Yi, Zhao and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Condition optimization and semi-quantitative analysis of recombinant proteins. (A) Western blot analysis of P97R1P46, P97R1P46-P65, P65-P42, and P97R1P46-P65-P42 proteins under different MOI and time conditions. (B) Semi-quantitative analysis of recombinant proteins. Lanes 1–6 are, in order, 800 μg/mL, 500 μg/mL, 250 μg/mL, 125 μg/mL, 62.5 μg/mL, and 31.25 μg/mL concentrations of standard protein; lane 7 is Ac-P65-P42 infected cell lysate; lane 8 is Ac-P97R1P46 infected cell lysate. Lane 9 is Ac-P97R1P46-P65 infected cell lysate. (C) Semi-quantitative analysis of recombinant proteins. Lanes 1–5 are, in order, 500 μg/mL, 250 μg/mL, 125 μg/mL, 62.5 μg/mL, and 31.25 μg/mL concentrations of standard protein; lane 6 is Ac-P97R1P46-P65-P42 infected cell lysate.