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Cytokine assays and immune cell assays in mouse immunoassays. (A) Detection of IL-2 in mouse serum. (B) Detection of IL-4 in mouse serum. (C) CD4+ T lymphocyte levels in the spleen of mice. (D) CD8+ T lymphocyte levels in the spleen of mice. Two-way ANOVA: *: p < 0.05, **: p < 0.01; ns: p > 0.05 (no significant difference).
