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Primers used for the pyrosequencing assay
| Gene | Amplification site | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | Sequencing primer (5′ to 3′)a |
|---|---|---|---|---|
| NFKB2 | −1281 to −1018 | GGGTTGGTTGAGTTAGTTTAGAGTTAAAT | Biotin-CCTCCTCCCTCTTTTCTCTTATCC | −1262-AGAGTTAAATTTTTAGTTAATGAA |
aThe sequencing primer was designed to analyze sense DNA
