Table 4.
Characterization of E. coli isolates grouped on profil II in terms of phenotypic resistance and its genotypic basis, phylogenetic groups, and clonal relatedness.
| Strains | Qs-FQs resistance phenotypes NAL NXN OFX CIP |
Qs -FQs MICs (mg/L) | ATB resistance profiles | QRDRs mutations | PMQRs gene | Phylogenetic group | ST131 clone | PFGE pulsotype/subtype |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| NAL S≤16 R>16 |
OFX S≤0.25 R>0.5 |
C.I.P. S≤0.25 R>0.5 |
ß-lactams | Aminoglycosides | Other | gyrA | parC | ||||||
| E. coli (7) | RSSS | 256 | 0.5 | 0.24 | AMP, TIC, PIP | - | - | S83L | WP | - | B2 | - | - |
| E.coli (8) | RSSS | 256 | 0.5 | 0.24 | AMP, TIC, PIP | - | SXT | S83T | WP | - | B2 | - | - |
| E. coli (9) | RSSS | 256 | 0.5 | 0.24 | - | - | SXT | S83L | WP | - | Clade I or II | - | - |
| E.coli (10) | RSSS | 256 | 0.5 | 0.24 | AMP, TIC, PIP | - | - | S83L | WP | - | B2 | - | - |
| E. coli (11) | RSSS | 256 | 0.5 | 0.24 | AMP, TIC, PIP | - | CHL, SXT | S83L | WP | - | B2 | - | - |
| E.coli (12) | RSSS | 256 | 0.5 | 0.24 | - | - | - | D87N | WP | - | B2 | + | Pulsotype VI |
| E.coli (13) | RSSS | 256 | 0.5 | 0.24 | - | - | - | D87N | WP | - | Unknown | - | - |
| E.coli (14) | RSSS | 256 | 0.5 | 0.24 | MEC | - | - | S83L | WP | - | Clade I or II | - | - |
| E. coli (15) | RSSS | 256 | 0.5 | 0.24 | AMP, TIC, PIP | - | SXT | S83L | WP | - | Clade I or II | - | - |
MIC: minimum inhibitory concentration; S: sensitive ; R: resistant; NAL: Nalidixic acid, NXN: Norfloxacin, OFX: ofloxacin; CIP: ciprofloxacin AMP: ampicillin; TIC: ticarcillin; PIP: piperacillin; MEC: mecillinam; CHL: chloramphenicol; SXT: co- trimoxazole; PMQR: plasmid resistance to fluoroquinolones, QRDR: quinolone resistance determining region, R: resistant; S: sensitive; WP: wild phenotype; +: positive; -: Negative.