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. 2024 Nov 26;15:10232. doi: 10.1038/s41467-024-54145-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 2 — Overview of the ST workflow: from patient to data analysis. ST analysis was conducted in triplicates on fresh-frozen surgical samples from 94 TNBC patients. Double CD3/CD20 IHC and bulk RNA sequencing were performed for each patient. Detailed morphological annotation was carried out on one of the available ST sections. Bioinformatic analyses integrated morphological features and ST sequencing data. Figure 2 was partly generated using Servier Medical Art, provided by Servier (https://smart.servier.com/), licensed under Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/). H/E hematoxylin and eosin, IHC immunohistochemistry, ST spatial transcriptomics, TNBC triple-negative breast cancer.