FIG. 2.

T-cell proliferative response of spleen cells from nonimmunized (injected with saponin alone) and immunized mice determined by [³H]thymidine incorporation on day 3 of culture. Data presented are means ± standard deviations for four individual mice separately assayed. Cytokine (IFN-γ and IL-4) production was determined in collected cultured supernatant obtained 72 h poststimulation. Data are the means for four mice and are representative of two experiments performed in duplicate. Significantly increased proliferative response and IFN-γ levels were achieved when cells from immunized mice were stimulated both with T. crassiceps antigens and GK-1 peptide.