TABLE 2.
Protective immunity against murine T. crassiceps cysticercosis by immunization with cysticercal antigens and different forms of GK-1
| Antigen(s) | Group | No. of cysticerci in each mouse in group
|
|
|---|---|---|---|
| 1st trial | 2nd trial | ||
| Linear GK-1 | Control | 4, 5, 5, 6, 7, 7, 9, 12 (0/8b) | 27, 24, 20, 21, 29, 20, 24, 27, 34 (0/9) |
| Immunized | 0, 0, 0, 0, 1, 2, 2c (4/7) | 0, 0, 0, 0, 0, 0, 0, 2, 5, 6c (7/10) | |
| MAP–GK-1 | Control | 15, 19, 24, 28, 51 (0/5) | 10, 12, 21, 26, 64 (0/5) |
| Immunized | 0, 0, 25, 40, 60, 62, 65, 75, 75, 79 (2/10) | 0, 0, 0, 0, 2, 17, 24, 27, 62, 81 (4/10) | |
| BSA–GK-1 | Control | 42, 50, 55, 57, 62 (0/5) | 7, 13, 17, 48, 53 (0/5) |
| Immunized | 9, 12, 14, 15, 36c (0/5) | 0, 0, 0, 0, 0, 0, 0, 0, 4, 23c (8/10) | |
| All T. crassiceps | Control | 13, 15, 16, 21, 23 (0/5) | 9, 14, 17, 19, 25 (0/5) |
| Immunized | 0, 0, 0, 0, 3, 5c (4/6) | 0, 0, 0, 0, 0, 2, 9c (5/7) | |
a
Mice were immunized twice with soluble T. crassiceps cysticercal antigens (100 μg per mouce), GK-1 free of carrier (10 μg per mouce), or BSA–GK-1 orMAP–GK-1 (each at 50 μg per mouce) in saponin. Control mice were injected twice with saponin in saline. Mice were challenged 15 days after the booster and sacrificed 30 days later. The parasite intensity in each mouse was determined. Data shown are from two independent experiments.
b
Number of mice without a single parasite of the total tested in each group.
c
Statistically significant difference between control and immunized mice at the 95% confidence interval.