TABLE 3.
Level of antibodies measured by ELISA during the immunization protocola
| Antibody against: | Mean antibody level (OD405) ± SD
|
||||
|---|---|---|---|---|---|
| Nonimmunized | Injected with:
|
||||
| Saponin (control)
|
Saponin + GK-1
|
||||
| 1st dose | 2nd dose | 1st dose | 2nd dose | ||
| GK-1 | 0.129 ± 0.007 | 0.146 ± 0.005 | 0.164 ± 0.010 | 0.164 ± 0.020b | 0.190 ± 0.020b |
| T. crassiceps antigens | 0.150 ± 0.01 | 0.189 ± 0.006 | 0.230 ± 0.027 | 0.247 ± 0.105b | 0.431 ± 0.131b |
a
Groups of 10 mice each immunized and tested 2 weeks after each immunization The serum antibody levels against GK-1 and T. crassiceps cysticercal antigens were determined by ELISA in GK-1-immunized, nonimmunized, and saponin-injected mice. Levels of murine antibodies raised during the course of immunization with GK-1 peptide are shown.
b
Considered statistically significant at P < 0.05.