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. 2024 Nov 18;31(2):31. doi: 10.3892/mmr.2024.13397

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Copyright: © 2024 Jiao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of low expression of PTEN on HUVECs treated with ox-LDL. (A) The expression of PTEN in HUVECs knocked down by si-PTEN detected by RT-qPCR. (B) The effect of PTEN knockdown on HUVECs proliferation detected by cloning assay. (C) The effect of PTEN knockdown on HUVECs migration detected by wound healing assay. (D) The effect of PTEN knockdown on HUVECs invasion detected by Transwell assay. (E) The effect of PTEN knockdown on HUVECs apoptosis detected by Flow cytometry. (F) The effect of PTEN knockdown on HUVECs angiogenic ability detected by tube formation assay. Magnification, 200×. **P<0.01. PTEN, phosphatase and tensin homolog; HUVECs, human umbilical vein endothelial cells; ox-LDL, oxidized low-density lipoproteins; si, short interfering; RT-q-PCR, reverse transcription-quantitative PCR; NC, negative control.