Figure 1.

Protective effect of selenium compounds on IEC‐6 cells and NCM‐460 cells against X‐rays. A,B) The survival rate of NCM‐460 and IEC‐6 cells after 48 h of X‐ray irradiation (0, 8, 16 and 32 Gy). Effects of X‐ray (16 Gy) and Se (2 µM) on the cell viability of NCM‐460 cells C) and IEC‐6 cells D). Cells were primed with Se for 6 h followed by X‐ray irradiation. After 48 h, cell viability was scrutinized by MTT assay (Data are represented as mean ± SD, n = 3). E‐F) Effects of Se and X‐ray irradiation on the cell apoptosis on IEC‐6 cells. Data are represented as mean ± SD, n = 2. Bars with characters a, b, and c are denoted as significant differences between the treatment and control groups. G) The colony formation of IEC‐6 cells after the therapy for Se (2 µM) and X‐ray (16 Gy). H‐I) Consequences of Se (2 µM) and X‐ray (16 Gy) on the mitochondria membrane potential of NCM‐460 and IEC‐6 cells. Cells were pre‐exposed to Se for 6 h and then irradiated by X‐ray. After 48 h, cells were assembled and stained with a JC‐1 probe and then analyzed by Flow cytometry assay. Data are represented as mean ± SD, n = 2. **P < 0.01 and **P < 0.001 levels are considered as significant differences in comparison with the untreated control group. Bars with characters a, b and c are denoted as significant differences between the treatment and control groups.