FIG. 5.
Receptor specificity of the wild-type-mediated inhibition. Time courses of [Ca2+]i in single neutrophils during infection with Y. pseudotuberculosis and subsequent stimulation with fMLP are shown. Adherent neutrophils were exposed to Yersinia plasmid-cured strain (A) or to the wild-type strain (B) at a calculated bacterium/cell ratio of 50:1 for 5 min. The “✗” symbols indicate contact between bacteria and the neutrophil. Neutrophils were then stimulated with 10−8 M fMLP (arrow). Representative time courses are presented (of three separate experiments).