FIG. 4.

In vivo expression of various cytokines and iNOS mRNA in Kurono strain-infected mice by RT-PCR. The spleen tissues of IL-18-KO (lanes 1, 3, 4, 6, 7, 9, 11, 13, and 15) and WT (lanes 2, 5, 8, 10, 12, and 14) mice were removed 7 weeks after infection. M, size marker; lanes: 1, IL-18 from IL-18-KO mouse (no amplified band); 2 to 4, TNF-α (no TNF-α in IL-18-KO mice treated with recombinant IL-18 [lane 4]); 5 to 7, iNOS (almost no amplified band in IL-18-KO mice treated with recombinant IL-18 [lane 7]); 8 and 9, IL-12; 10 and 11, IL-1β; 12 and 13, IFN-γ; 14 and 15, β-actin.