FIG. 4.

Effect of antichemokine Abs on macrophages infiltrating the brains of Hib-infected rats as shown by immunohistochemistry. Shown are representative fields (obtained 72 h p.i.) from animals treated with rabbit Ig as control Ab (a), rabbit polyclonal anti-MIP-2 (b), rabbit polyclonal anti-MIP-1α (c), and rabbit polyclonal anti-MCP-1 (d). (a) Control Ab-treated animals showed diffuse macrophage infiltration in the subarachnoid space. Anti-MIP-2 and anti-MIP-1α-treated animals exhibited less diffuse macrophage infiltration. Animals treated with anti-MCP-1 showed much reduced macrophage infiltration. Staining was performed on 10-μm-thick cryosections as described in Materials and Methods, with immunoperoxidase and hematoxylin. Magnification, ×400.