FIG. 6.

Effect of MIP-2, MIP-1α, and MCP-1 Abs on chemokine mRNA expression. Four groups of six infant rats received either anti-MIP-2, anti-MIP-1α, and anti-MCP-1 Ab (5 μg/day) or isotype control Ab (5 μg/day) i.p. 30 min before Hib inoculation. Rats then received i.p. injections with 100 μl containing 10⁵ CFU of Hib per ml. Results are given as mean numbers of cells expressing mRNA for MIP-2, MIP-1α, MCP-1, and RANTES per 100 mm² of surface area, detected by in situ hybridization as described in Materials and Methods (error bars, standard deviations). Brain sections were obtained from anti-MIP-2-treated and isotype control-inoculated groups at 72 h and 7 days p.i. For each panel, the significance of differences between treated and untreated groups of Hib-inoculated rats was determined by Student’s t test. ∗∗∗, P < 0.001; ∗∗, P < 0.01; ∗, P < 0.05.