Skip to main content
. 2024 Jul 23;59(12):3221–3227. doi: 10.1002/ppul.27178

Figure 3.

Figure 3

In vitro effects of CsA and HCQ on ABCA3 function of G210C and Q1045R. All cells were incubated with RPMI‐1640 + 10% fetal bovine serum (FBS). Ctr (abbreviation for blank control): 0.1% dimethylsulfoxide (DMSO); C: CsA 10 μM; H: HCQ 10 μM; C + H: CsA 10 μM + HCQ 10 μM. Results were expressed as means ± SEM. (A) % WT‐like cells: experiments done by machine, nSD value of WT DMSO: 12.58% (n = 2). (B) % lysosome localization*: experiments done manually, nSD value of WT DMSO: 9.8% (n = 60 ABCA3+ vesicles). (C) Proteolytic cleavage: experiments done manually, nSD value of WT DMSO: 17.59% (n = 3). (D) Volume of ABCA3+ vesicles*: experiments done manually, nSD value of WT DMSO: 10.22% (n = 3). (E) TopF‐PC transport activity (intensity)*: experiments done manually, nSD value of WT DMSO: 53.82% (n = 3). (F) TopF‐PC transport activity (% filled)*: experiments done manually, nSD value of WT DMSO: 35.09% (n = 3). n: number of independent experiments. *60 ABCA3+ vesicles were randomly selected and analyzed from 3 different images in each independent experiment. Black dotted line: mean value; green dotted line: ±1 nSD; red dotted line: ±3 nSD. CsA, cyclosporine A; HCQ, hydroxychloroquine; nSD, normalized standard deviation; SEM, standard error of mean.