TABLE 1.
Characteristics of the primers used in this study
| Name | Gene regiona | Strand | Nucleotide sequence | Source or reference |
|---|---|---|---|---|
| Par1 | parBS | + | ggcgtcaatggttgagatgact | 3 |
| Par2 | parBS | − | gtccagttcatcctgaaccact | 3 |
| IncR1 | incR, parA | + | agcacgtttgacagggtaacg | 3 |
| IncR2 | incR, parA | − | gtggcgactttccgtaactgct | 3 |
| SpvR | spvRA | + | aacaccatgattagtaagaactaatcagt | 15 |
| SpvA | spvRA | − | cctgaacaatgacgtcgctcagat | 15 |
| SpvC1 | spvC | + | cttgcacaaccaaatgcggaagat | 15 |
| SpvC2 | spvC | − | ctctgcatttcaccaccatcacg | 15 |
| Rsk1 | rsk | + | ccctacccaggtgttgaagtcat | 30 |
| Rsk2 | rsk | − | ccttctccctctcagcagcttcat | 30 |
| RepA1 | repA (RepFIB) | − | gaaccggcaaggaagcgcaatgt | 25 |
| RepA2 | repA (RepFIB) | + | ccctacccaggtcttgaaatcgt | 25 |
| PefA | pefAC | + | ccgaaggtgacttcaagtctgt | 6 |
| PefC | pefAC | − | cggcatttgcataggcactggt | 6 |
| PefD1 | pefD, orf5 | + | gcagcagtacggtgtatatggt | 6 |
| PefD2 | pefD, orf5 | − | cctccggtgaattttgccggaat | 6 |
| Rck1 | rck | + | tcgttctgtcctcactgctgct | 9 |
| Rck2 | rck | − | accggtaaccgacaccaacgtt | 9 |
| RepB1 | repA (RepFIIA) | + | ccctgccgttctgtcgtaagct | 25 |
| RepB2 | repA (RepFIIA) | − | tggtaggtaatcagccccagct | 25 |
| TraX-1 | traX, finO | + | aaccgtggcgctgctgctgat | This study |
| TraX-F | traX, finO | − | cttccacttcgggggcgtggt | This study |
| TraT-1 | traT | + | ggttacactggtcagttccactct | 24 |
| TraT-2 | traT | − | gccagttgttcttccagaactggt | 24 |
| Spt5 | oriT | + | ggttacgggattccttccatgaaat | This study |
| TraM-F2 | oriT | − | atatctttatctctcgccccttcct | This study |
| Sam1 | samA | + | gaggaactggatctgaatgcct | 20 |
| Sam2 | samA | − | gatttcctccaccggttgcagt | 20 |
a
parBS, parB-parS; pefAC, pefA-pefC; spvRA, spvR-spvA.