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. 2005 Jun 27;33(Web Server issue):W489–W492. doi: 10.1093/nar/gki358

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© The Author 2005. Published by Oxford University Press. All rights reserved

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SNP Cutter workflow. Rectangles and rhombi show the task that the program performs. It includes five steps, shown as Step 1 through 5 in the figure. Parallelograms show the data that the user supplies or every step produces. The program produces five files as shown. ‘(tag.)sc.xxxx_DNinput.txt’ is the output of Step 1 SNPSequer; ‘(tag.)sc.xxxx_PRI3.txt’ is the output of Step 3 of Primer3 analysis; ‘(tag.)sc.xxxx_detail.txt’ and ‘(tag.)sc.xxxx_rflp.txt’ are the outputs of Step 5. ‘(tag.)sc.xxxx_failed.txt’ logs all SNPs failed at assay design. ‘RE’ is the abbreviation for ‘Restriction Enzyme.’ ‘(tag.)’ is an optional string user provides as identifier in the filename. ‘xxxx’ is a random number that the system produces.