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[Preprint]. 2024 Nov 15:2024.11.13.623456. [Version 1] doi: 10.1101/2024.11.13.623456

Figure 1. Pcgf2 is required for neuronal differentiation.

Figure 1.

a, Heat-map of differentially expressed genes (DEGs) in duplicated WT and Pcgf2−/− NPCs, clustered and sorted based on transcript z-score. A total of 2,263 upregulated and 1,576 downregulated genes were identified. b, Gene ontology (GO) analysis for downregulated genes in Pcgf2−/− NPCs from (a). c, Immunoblotting of cPRC1 components (Phc1, Pcgf2, Pcgf4, and Ring1b) and H2AK119ub1 in WT and Pcgf2−/− ESCs. d, ChIP-seq heatmaps showing H2AK119ub1 and Ring1b enrichment in WT and Pcgf2−/− ESCs at Ring1b (PRC1) target loci. e, ChIP-seq tracks for Ring1b, H2AK119ub1, H3K4me3, and H3K27me3 in WT and Pcgf2−/− ESCs and RNA-seq tracks for WT and Pcgf2−/− ESCs and NPCs at PRC1 target loci (Hoxa, Irx3/Irx5, and Foxf2/Foxc1). f, Box plots of expression levels for all PRC1.2 target genes (5,334), bivalent PRC1.2 target genes (3,284), and non-bivalent PRC1.2 target genes (2,050). ***p<0.001; n.s., not significant.