Figure 4. Deleting Phc1 SAM domains compromises target gene activation in NPCs.

a, Schematic of Phc1 SAM domain deletion (Phc1ΔSAM) for disrupting cPRC1 oligomerization. b, Immunoblotting of Phc1, Ring1b, Rybp, Pcgf2, and H2AK119ub1 in WT and Phc1ΔSAM ESCs. Note the smaller size of the Phc1 band in the Phc1ΔSAM sample. c, RT-qPCR analysis showing failed induction of NPC markers (Pax6 and Nes) in WT and Phc1ΔSAM NPCs. n = 3 for each sample. d, Principal component analysis (PCA) of RNA-seq data from WT, Phc1^−/−, and Phc1ΔSAM ESCs and NPCs. e, Box plots of expression of neuro-ectodermal and non-neuro-ectodermal genes targeted by cPRC1.2 loops in WT and Phc1ΔSAM NPCs. **p<0.01; n.s., not significant.