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[Preprint]. 2024 Nov 15:2024.11.13.623456. [Version 1] doi: 10.1101/2024.11.13.623456

Figure 5. CTCF is colocalized at cPRC1.2 sites.

Figure 5.

a, ChIP-seq enrichment of Ring1b, Pcgf2, and CTCF at cPRC1.2 loop anchors with 0.2 Mb regions flanking each anchor in WT ESCs. b, Immunofluorescent images of U2OS cells co-stained for CTCF and RING1B (top panel) and PCGF2 and RING1B (bottom panel). c, Quantification of CTCF fluorescence within or outside RING1B puncta (n = 50). d, Immunoblotting of RING1B and CTCF in RING1B knock-down (siRING1B) and control (siControl) U2OS cells. e, Immunofluorescent images of control (siControl) and RING1B knock-down (siRING1B) U2OS cells showing a reduction in CTCF puncta with RING1B knockdown. White circular outlines indicate the nuclei boundaries for cells with successful knockdown. f, Dot plots showing the number of RING1B and CTCF puncta in siControl (n = 54) and siRING1B (n = 50) U2OS cells. ***p<0.001.