Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The assembly PCR method for constructing long DNA molecules. (a) In the first PCR step a pool of oligodeoxynucleotides anneal and are (b) elongated to produce a full-length DNA molecule. In addition to the full-length product, a host of shorter molecules also results. (c) In the second PCR step the desired full-length molecule is selectively amplified from the mixture using primers specific for the desired full-length product.
