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. 2005 Jun 27;33(Web Server issue):W521–W525. doi: 10.1093/nar/gki380

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© The Author 2005. Published by Oxford University Press. All rights reserved

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The construction of a 191-nt DNA molecule using the oligodeoxynucleotides determined by the Assembly PCR Oligo Maker program. (a) The sequence of the 191-nt DNA target to be produced. (b) The DNA sequences reported by Assembly PCR Oligo Maker program. Sequences for both steps of the assembly PCR reaction are reported. (c) Diagram showing how the four oligodeoxynucleotides anneal to produce the full-length dsDNA product (d) Agarose gel showing the results of the first (lane 2) and second (lane 3) PCR steps. The desired 191-nt molecule is visible after the second PCR step. DNA length markers are shown in lane 1.