Table 1. Overview of Studies Included in the Meta-Analysis.
Within the table, “GEO ID #” refers to the Gene Expression Omnibus (GEO) accession number for the dataset. The sample size (“n”) for the full dataset is provided, as well as the final sample size for the control (“Ref”) and ELS groups included the subset of the dataset used for our analysis (final total n: Ref=42, ELS=47). The subsetted sample size only includes the ROI (prefrontal cortex (PFC) or medial PFC (mPFC)) and excludes outlier samples. Please note that each sample in GSE89692 represents RNA pooled from 3 subjects. The Author and Year refer to the publication (Benekareddy et al., 2010; Oldham Green et al., 2021; Peña et al., 2017; Rincel et al., 2019; Zheng et al., 2019). “Duration” indicates how long (hours: “hrs”) the pups experienced maternal separation each day; limited nesting and bedding lasted all day. The “P” under “Age” represents the postnatal days during which the rodents were stressed. In GSE116416, the ELS group also received a prenatal immune challenge of lipopolysaccharide (LPS) on embryonic day 17 (E17). “Age Tissue” indicates the age of sacrifice, when the tissue from the ROI was collected. All experimental details refer specifically to the samples used in the transcriptional profiling experiment and not the full paper.
| GEO ID # | n (Full Dataset) | # Outliers | Final n (Ref) | Final n (ELS) | Species | Author | Year | Platform | Tissue | Type | Duration | Age Stressed | Age Tissue | Sex |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GSE89692 | 163* | 0 | 12 | 11 | Mouse | Pena et al. | 2017, 2019 | Illumina (RNA-seq) | mPFC | Maternal Separation & Limited Nesting | 4 hrs/day Separation | P10 – 20 (Male); P10 – 17 (Female) | Adult | Both |
| GSE116416 | 47 | 2 | 20 | 24 | Mouse | Rincel et al. | 2019 | Agilent (Microarray) | mPFC | Maternal Separation, Maternal Stress, & Prenatal LPS | 3 hrs/day Separation | P2 - P14; Prenatal LPS: E17 | Adult | Both |
| GSE14720 | 11 | 0 | 3 | 4 | Rat | Benekareddy et al. | 2010 | Agilent (Microarray) | PFC | Maternal Separation | 3 hrs/day Separation | P2 - P14 | Adult | Male |
| GSE153043 | 10 | 1 | 4 | 5 | Rat | Green et al. | 2021 | Illumina (RNA-seq) | PFC | Limited Nesting | P2-P9 | Adult | Male | |
| GSE124387 | 9 | 0 | 3 | 3 | Rat | Zheng et al. | 2019 | Illumina (RNA-seq) | PFC | Maternal Separation | 4 hrs/day Separation | P1 - P21 | Adult | Male |