Figure 6. Immortalized Carollia cells support whole virus replication.
(A) Primary and immortalized kidney (CaPsm-K) cells were infected with VSV-GFP (MOI 1), MERS-CoV (MOI 0.1), or SARS-CoV-2 (MOI 0.01) for up to 72 hours. Replication was monitored using TCID50 limiting dilution assays. (B) Clonal transduced kidney cells (CKg9) cells and CaPsmK-SV40T cells infected with Andes virus were monitored by qPCR. (C) CKg9 cells infected with Seoul virus (SEOV) were monitored by end-point microscopy for Seoul nucleocapsid protein.
