Figure 3. Fc effector functions are required for SKT20 protection but dispensable for SKT05-mediated survival during VEEV challenge.

(A) Neutralization curve for wild-type and LALA-PG variants against VEEV Env-pseudotyped virus. Data is representative of one independent experiment, conducted in triplicate. (B) Binding of indicated mAbs to purified human (h) FcγRI was assessed by ELISA. The data is represented as the mean ± SD of two independent experiments performed in duplicate. (C-F) Indicated mAbs (200 μg) were administered to C3H/HeN mice 1 day prior to inoculation with TC-83. Mice were followed for weight loss (mean ±SD) (C, D) and survival (E, F) for 13–14 days (n = 8/group; 2 independent experiments). Statistical significance was determined by a one-way ANOVA with a Tukey’s post-test of AUC analysis from 0–7 dpi comparing all groups (C and D) or a Log-rank test (E and F).