FIG. 2.

Neutralization of rSm28GST enzymatic activity by specific antisera. Neutralizing activity was analyzed for sera obtained 10 weeks after a single intranasal (A) or intragastric (B) administration of either rSm28GST-entrapped PLG or PCL microparticles, free rSm28GST, or empty microparticles. The results were expressed as the mean values ± standard deviations S.D. of the neutralizing activity (percent) of sera for five mice in each group. The catalytic neutralization of rSm28GST (3.5 pmol/well) was measured in the absence or presence of increasing concentrations of antisera. Sera expressing less than 10% of neutralization at a concentration of 0.2 μl/well are considered inefficient sera. Results are from one representative experiment of three.