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. 2024 Dec 2;134(23):e181044. doi: 10.1172/JCI181044

Figure 2. HO-1 inhibits RT-mediated IFN-I production.

Figure 2

(A) ELISA for IFN-β content in the supernatant of control or HMOX1-KO cells before and after RT. (B) Typical IFN-Is and ISGs mRNA levels of control or HMOX1-KO cells before and after RT. (C) Tumor growth of HMOX1-inducible knockdown HK1 tumors in HuHSC-NCG mice, following with or without RT (10 Gy) (n = 5 in each group). (D–F) RT-qPCR analysis for mRNA levels of typical IFN-I and ISG genes (D) and flow cytometric analysis of CD8+ T cell infiltration (E) and TNF-α and IFN-γ expression of CD8+ T cells (F) in the HK1 model (n = 5 in each group). APC, allophycocyanin. (G) Ifnb1, Ifna4, and Cxcl10 mRNA levels in BMDMs from Hmox1fl/fl and Hmox1fl/fl LyzCre/Cre mice. BMDMs were infected with HSV-1 or VSV. Data are shown as the mean ± SD (A, B, and D–G) and the mean ± SEM (C). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, by 1-way ANOVA (A, B, D, and E), 2-way ANOVA (C), and unpaired, 2-tailed, Student’s t test (F and G). n = 3 biologically independent experiments, unless otherwise stated. Dox, doxycycline.