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. 2024 Dec 2;134(23):e181044. doi: 10.1172/JCI181044

Figure 3. HO-1 inhibits the activity of cGAS and STING under RT independent of its enzymatic activity.

Figure 3

(A) Immunoblot analysis of essential molecules in IFN-I signaling from control or HMOX1-KO cells before and after RT. (B) ELISA of cGAMP production of control or HMOX1-KO cells before and after RT. (C) ELISA of IFN-β production in the supernatant of control or HMOX1-KO cells with or without cGAMP stimulation. (D and E) Immunoblot analysis of the indicated proteins from control or HMOX1-KO cells with the indicated treatment. (F and G) ELISA of cGAMP (F) or IFN-β (G) production in HK1 cells treated with the indicated metabolites. (H and I) HMOX1-KO HK1 cells were stably transfected with WT HO-1 or HO-1H25A. ELISA of cGAMP (H) or IFN-β (I) production with or without the indicated stimulation. Data are shown as the mean ± SD. ***P < 0.001 and ****P < 0.0001, by 1-way ANOVA (B, C, and F–I). n = 3 biologically independent experiments. p-, phosphorylated.