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. 2024 Nov 22;9(22):e171585. doi: 10.1172/jci.insight.171585

Figure 9. Decreased MUC5B sulfated O-glycans in LSGs from patients with SjD.

Figure 9

(A–F) Representative images of immunohistochemical detection of partially glycosylated MUC5B (PANH2) (A and D), the MUC5B polypeptide backbone independent of its glycosylation status (EU-MUC5Bb) (B and E), and sulfo-Lewis a and c residues (F2) (C and F) in serial sections of LSGs from individuals acting as controls (A–C) and patients with SjD (D–F). Scale bars: 50 μm. (G) Spearman’s correlation between Gal3STs activity and the percentage of sulfo-Lewis a and c (F2)-positive acini. *P < 0.05 was considered significant. (H) Oligosaccharide mixtures of MUC5B obtained by reductive β-elimination analyzed by HPAEC-PAD in LSGs from individuals acting as controls (black line) and patients with SjD (blue line). The asterisk in H shows a peak detected in control samples but not in SjD samples. nC, chromatographic fingerprint of the signal.