Figure 2. Treg-specific deletion of Ku70 enhances T cell activation in spleen and pLN.

(A) Representative flow cytometry staining (left) for CD62L and CD44 on the surface of different subpopulations of T cells in spleen and pLNs of WT and cKO mice (14 weeks old, n = 4) and summaries (right) for the frequencies of the activation status of T cells are shown. (B) Frequency of Tregs and IFN-γ–producing CD4⁺ and CD8⁺ T cells in different organs in WT and cKO mice (14 weeks old, n = 4). (C) Frequency of Tregs and IFN-γ–producing CD4⁺ and CD8⁺ T cells in different organs in WT and cKO mice (30 weeks old, n = 4). (D and E) Tregs were sorted from the spleens of WT and cKO mice. Rag^–/– mice (n = 4) were injected with 0.45 × 10⁶ naive CD4⁺CD45.1⁺ T cells alone or coinjected with 0.15 × 10⁶ Tregs from WT and cKO mice. Mouse body weight was measured weekly (D), and frequency of IFN-γ–producing CD45.1⁺CD4⁺ T cells and mean fluorescence intensity (MFI) of IFN-γ in different organs in recipient mice was also measured (E). Data are representative of 3 independent experiments. Data are represented as means ± SD. Significance was measured by unpaired 2-tailed Student’s t test.