Figure 3. The absence of Ku70 in Tregs unleashes potent pulmonary antitumor immune responses.

(A) Macroscopic evaluation of tumor size in lung from LLC-bearing WT and cKO mice (n = 6) at 21 days after tail-vein injection. (B) Representative lung histopathology images and percentage of lung area occupied by tumor lesions based on lung histopathological analysis (n = 5). (C and D) Representative histogram of CD4⁺ and CD8⁺ T cells (C) in lung cancer and summaries (D) for the frequencies (n = 6). (E) Frequency of IFN-γ– and TNF-α–producing CD8⁺ T cells in tumor of WT and cKO mice (n = 5). (F) Representative histogram of Treg proportion (left) in lung cancer and summaries (right) for the frequencies and Foxp3 MFI (n = 6). (G and H) Representative histogram of CD4⁺ and CD8⁺ T cells (G) in dLNs and summaries (H) for the frequencies (n = 6). (I) Frequency of TNF-α– and IL-2–producing Tconv cells in dLNs of WT and cKO mice (n = 6). (J) Representative histogram of Treg proportion (left) in dLNs and summaries (right) for the frequencies and Foxp3 MFI (n = 6). Data are represented as means ± SD. Significance was measured by unpaired 2-tailed Student’s t test.