Figure 4. Tregs with Ku70 depletion trigger robust immune responses to restrain melanoma lung metastasis.

(A) Macroscopic evaluation of tumor size in lung from B16F10-bearing WT and cKO mice (n = 5) at 21 days after tail-vein injection. (B and C) Representative histogram of CD4⁺ and CD8⁺ T cells (B) in lung cancer and summaries (C) for the frequencies (n = 5). (D) Frequency of IFN-γ– and GZMB-producing CD8⁺ T cells in tumor of WT and cKO mice (n = 5). (E) Representative histogram of Treg proportion (left) in lung cancer and summaries (right) for the frequencies and Foxp3 MFI (n = 5). (F) Representative histogram of CTLA4 expression (left) in lung cancer and proportion summaries (right) for CTLA4⁺ Tregs in tumor (n = 5). (G) Representative histogram of CD4⁺ and CD8⁺ T cells in tumor dLNs and summaries for the frequencies (n = 5). (H) Representative histogram of Treg proportion (left) in dLNs and summaries (right) for the frequencies and Foxp3 MFI (n = 5). Data are represented as means ± SD. Significance was measured by unpaired 2-tailed Student’s t test.