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. 2024 Oct 9;52(21):13138–13151. doi: 10.1093/nar/gkae874

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — DSF analyses of the stabilizing effect of DNA/RNA aptamers on N-NTD. (A) Schematics of various stem-loop aptamers. Sequence modifications from the parental A58-20 are highlighted in bold. For each aptamer, the T_m value of N-NTD in its presence is shown on the right (the shift of T_m from that for N-NTD alone is shown in parenthesis). (B) Melt curves of N-NTD in the presence of indicated aptamers. The control sample (black trace) contained no aptamer. Identical T_m values were obtained in duplicate experiments, from which one melt curve for each sample is shown.