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Comparison of the enzymatic properties of the wild-type rAprY and the single point mutants.
| Wild-type rAprY | A216E | A216K | A216R | |
|---|---|---|---|---|
| Km (μmol/L) | 1.52 ± 0.08 | 2.44 ± 0.12 | 1.29 ± 0.04 | 2.27 ± 0.36 |
| kcat (S−1) | 7.10 ± 0.69 | 9.21 ± 0.32 | 5.53 ± 0.16 | 20.38 ± 0.33 |
| Kd (kcat/Km) | 4.67 | 3.77 | 4.28 | 8.97 |
| Specific activity (U/mg) | 398.27 ± 35.6 | 428.98 ± 25.4 | 483.15 ± 50.6 | 436.40 ± 18.9 |
| half-life (min) | 9.80 ± 0.68 | 54.30 ± 1.2 | 55.93 ± 0.98 | 35.36 ± 0.96 |
| Optimal pH | 8 | 8 | 8 | 8 |
| Optimum temperature (°C) | 54 | 56 | 56 | 56 |
