Table 1.
Cell cluster identification.
| MC | Population | Subset | Phenotypic expression | Functional expression |
|---|---|---|---|---|
| 1 | CD4+ | Naive | CD3, CD4, CD27, CD28, CD127, CD45RA | CCR7 |
| 2 | CD4+ | Early effector (1) | CD3, CD4, CD27, CD28, CD2dim, CD25dim | |
| 3 | CD4+ | Early effector (2) | CD3, CD4, CD27, CD28, CD2dim, CD127 | CXCR3, FASdim |
| 4 | CD4+ | Early like effector (1) | CD3, CD4, CD28, CD2 | HLADRdim |
| 5 | CD4+ | Early like effector (2) | CD3, CD4, CD28, CD2, CD127 | Fasdim |
| 6 | CD4+ | Terminal effector (1) | CD3, CD4, CD2 | HNK1dim, FASdim |
| 7 | CD4+ | Terminal effector (2) | CD3, CD4, CD2 | CCR5dim |
| 8 | CD4+ | Central memory (1) | CD3, CD4, CD27dim, CD28, CCR7dim, CD2dim, CD39, CD25dim, CD14dim, CD38 | NKG2C, HLADRdim |
| 9 | CD4+ | Central memory (2) | CD3, CD4, CD27, CD28, CCR7dim, CD127 | |
| 10 | CD4+ | Central memory (3) | CD3, CD4, CD27, CD28, CCR7dim, CD127 | CXCR3 |
| 11 | CD8+ | Naive | CD3, CD8, CD27, CD127, CD45RA, CCR7 | CXCR3, NKG2Ddim |
| 12 | CD8+ | Early effector | CD3, CD8, CD27dim, CD28, CD2, CD127 | FASdim |
| 13 | CD8+ | Early like effector | CD3, CD8, CD28, CD2, CD127 | |
| 14 | CD8+ | Intermediate effector | CD3, CD8, CD27dim, CD2, CD127 | NKG2Ddim, CXCR3, FASdim |
| 15 | CD8+ | Terminal effector (1) | CD3, CD8,CD2dim | NKG2Ddim |
| 16 | CD8+ | Terminal effector (2) | CD3, CD8, CD2 | NKG2Ddim, HNK1dim |
| 17 | CD8+ | TEMRA (1) | CD3, CD8, CD2dim, CD45RA | NKG2D |
| 18 | CD8+ | TEMRA (2) | CD3, CD8, CD2, CD45RA | NKG2Ddim, HNK1dim |
| 19 | CD8+ | CD45RA llike Terminal effector | CD3, CD8, CD2dim, CD45RAdim | NKG2A, NKG2Ddim, HNK1dim |
| 20 | CD4+/CD8+ T cells | CD3, CD4, CD8, CD27dim, CD28, CD2, CD127 | FASdim | |
| 21 | CD4-/CD8- T cells | (1) | CD3, CD27dim, CD28dim, CD127 | CCR5dim, CXCR3dim |
| 22 | CD4-/CD8- T cells | (2) | CD3, CD27, CD28dim, CD38, CD2dim | CCR5dim, FAS, HLADRdim |
| 23 | Tγδ | TEMRA (1) | CD3, TCRγδ, CD45RA | |
| 24 | Tγδ | TEMRA (2) | CD3, TCRγδ, CD45RA, CD2dim, CD8 | NKG2D, FAS, PD1dim |
| 25 | Tγδ | TEMRA (3) | CD3, TCRγδdim, CD45RA, CD2dim | HNK1dim |
| 26 | Tγδ | TEMRA (4) | CD3, TCRγδ++, CD45RA, CD2dim, CD56 | NKG2D, NKG2A |
| 27 | Tγδ | TEMRA (5) | CD3, TCRγδ++, CD45RA, CD2dim, CD56 | NKG2D, NKG2A, CXCR3dim, HNK1 |
| 28 | Tγδ | TEMRA (6) | CD3, TCRγδ, CD45RA, CD2dim, CD127, CD27dim | CXCR3, NKG2D, NKG2A |
| 29 | Tγδ | TEMRA (7) | CD3, TCRγδdim, CD45RA, CD2dim, CD127, CD27dim | CXCR3 |
| 30 | Tγδ | CD45RAdim/CCR7- | CD3, TCRγδ, CD45RAdim, CD2dim | NKG2A, NKG2D, HNK1dim |
| 31 | Tγδ | Effector memory (1) | CD3, TCRγδdim, CD27, CD127, CD28, CD2 | CXCR3, NKG2Ddim, CCR5dim, NKG2A |
| 32 | Tγδ | Effector memory (2) | CD3, TCRγδdim, CD27, CD127, CD2dim | CXCR3, NKG2Ddim, CCR5dim |
| 33 | Treg | CD45RA-/CD39+ | CD3, CD4, CD27, CD28, CD25, CD39, CD2 | FAS, NKG2C, HLADRdim, CXCR3, NKp30, CCR5 |
| 34 | B cells | Memory | CD19, CD20, CD27, CD39, CD24, CD25, CD2, CD45RA, CCR7 | CXCR3dim, NKG2C, CCR6, CXCR5, NKp30, HLA-DR |
| 35 | B cells | No memory (1) | CD20dim, CD28dim, CD39dim, CD2dim, IgD, CD45RA, HLA-DR | NKG2C, CXCR5, HLA-DRdim |
| 36 | B cells | No memory (2) | CD19dim, CD20dim, CD28dim, CD39dim, CD2dim, IgD, CD45RAdim, CD24, CCR7, CD25dim | NKG2C, CXCR5, HLA-DRdim, CXCR3 |
| 37 | B cells | Plasmablasts | CD19, CD27, CD39, CD24, CD25, CCR7 | NKG2C, CXCR5, HLADR |
| 38 | Unknown myeloid antigen presenting cells | CD45RA, CD16, CD39, CD11c, CD123, CD141, TCRγδdim, CD127, CD20dim | NKG2C, FASdim, HLA-DRdim, CXCR3 | |
| 39 | Mixed myeloid antigen presenting cells | CD45RA, CD16, CD39, CD11c, CD123, CD141 | NKG2C, FASdim, HLA-DRdim | |
| 40 | Monocytes | Classic (1) | CD14, CD38, CD39dim | FAS, NKG2C, HLA-DRdim, CXCR5 |
| 41 | Monocytes | Classic (2) | CD14, CD38, CD39, CD123, CD141, CD25, CD2, CD127, CD4dim | FAS, NKG2C, HLA-DR, NKp30 |
| 42 | Monocytes | Non-classical | CD16, CD39dim, CD123, CD141, CD25, CD2, CD11c++, CD1c, CD20dim, CD45RA | FAS, NKG2C, HLA-DR, NKp30, NKG2D |
| 43 | NK | Early NK | CD56, CD45RA, CD38 | |
| 44 | NK | Mature NK (1) | CD56, CD45RA, CD38, CD16, CD2dim | HNK1dim, NKG2Ddim, NKG2Adim |
| 45 | NK | Mature NK (2) | CD56, CD45RA, CD38, CD16, CD2dim | HNK1dim, NKG2Ddim, NKG2C |
| 46 | ILCs | Total ILCs CD2- | CD56, CD38, CD127, CD45RA | CXCR3 |
| 47 | ILCs | CD4- (1) | CD56, CD38, CD127, CD39, CD2, CD45RA | CXCR3, NKG2A, NKG2Ddim, NKp30dim |
| 48 | ILCs | CD4- (2) | CD56, CD38, CD127, CD39, CD2 | CXCR3, NKG2A, NKG2Ddim, NKp30dim |
For each of the 48 identified FlowSOM clusters (C), the cell population to which it belongs is shown, alongside the specific subset, phenotype and expression of functional markers. Markers highlighted in bold denote differential expression within the same population.