Figure 4.

Fc.Mut24 increases CTLA-4-dependent transendocytosis of CD80 and CD86 by Foxp3⁺ T_R cells

(A) Experimental design schematic for ex vivo CTLA-4 transendocytosis assay. Gates were set on live, CD4⁺, Foxp3⁺ T_R, or Foxp3⁻ T_conv cells.

(B) Capture of CD86-GFP by Foxp3⁺ T_R and Foxp3⁻ T_conv between different treatment groups. Representative flow-cytometry plots for T_R are shown. Graph shows mean ± SD with individual data points (n = 7); ^∗∗∗∗p ≤ 0.0001, multiple unpaired t tests. Data are representative of 2–3 independent experiments.

(C) Capture of CD86-GFP by Foxp3⁺ T_R between different co-culture conditions. Representative flow-cytometry plots from Fc.Mut24-treated mice are shown. Graph shows mean ± SD with individual data points (n = 7); ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001, ^∗∗∗∗p ≤ 0.0001, multiple paired t tests. Data are representative of 2–3 independent experiments.

(D) Experimental design schematic for in vivo CTLA-4 transendocytosis assay. Gates were set on CD4⁺, Foxp3⁺ T_R, or Foxp3⁻ T_conv.

(E) Capture of CD80-mCherry by Foxp3⁺ T_R and Foxp3⁻ T_conv between different treatment groups. Representative flow-cytometry plots are shown. The graph shows Foxp3⁺ T_R data, and mean ± SD with individual data points (n = 5 per group); ^∗p ≤ 0.05, ^∗∗∗∗p ≤ 0.0001, multiple unpaired t tests. Data are representative of three independent experiments.