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. 2024 Nov 2;43(11):114938. doi: 10.1016/j.celrep.2024.114938

Figure 5.

Figure 5

Fc.Mut24 promotes CTLA-4-dependent transendocytosis of CD80 and CD86 during dendritic cell maturation

(A) Experimental design schematic for the in vivo dendritic cell (DC) maturation model. See Figure S5A for the gating strategy.

(B–E) Expression of indicated DC maturation markers. Representative histograms are shown. Graphs show mean ± SD with individual data points (n = 7–10); ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, multiple unpaired t tests. Ctrl, control. For MHC class II expression, data are shown as fold-change geometric MFI over the mean of PBS control (dotted line). Data are representative of 2–3 independent experiments.

(F) Experimental design schematic for the anti-CTLA-4 study.

(G) Expression of indicated DC maturation markers between isotype- and anti-CTLA-4 antibody-treated mice. Graphs show mean ± SD with individual data points (n = 7); ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001, multiple unpaired t tests. n.s., not significant. Data are representative of 2–3 independent experiments.