REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-mouse CD4 BV650 (RM4-5)	BioLegend	Cat# 100555; RRID: AB_2562529
Anti-mouse CD4 BV421 (RM4-5)	BioLegend	Cat# 100563; RRID:AB_2563052
Anti-mouse CD45 BUV395 (30-F11)	BD Biosciences	Cat# 564279; RRID:AB_2651134
Anti-mouse TCRβ BUV737 (H57-597)	BD Biosciences	Cat# 612821; RRID:AB_2870145
Anti-mouse CD25 BV785 (PC61)	BioLegend	Cat# 102051; RRID: AB_2564131
Anti-mouse Foxp3 PE (FJK-16s)	eBioscience (ThermoFisher)	Cat# 12-5773-82; RRID:AB_465936
Anti-mouse Foxp3 eFluor450 (FJK-16s)	eBioscience (ThermoFisher)	Cat# 48-5773-82; RRID:AB_1518812
Anti-mouse Ki-67 AF488 (11F6)	BioLegend	Cat# 151204; RRID:AB_2566800
Anti-mouse CD44 AF700 (IM7)	BioLegend	Cat# 103026; RRID:AB_493713
Anti-mouse CD44 BV510 (IM7)	BioLegend	Cat# 103043; RRID:AB_2561391
Anti-mouse CD62L BV711 (MEL-14)	BioLegend	Cat# 104445; RRID:AB_2564215
Anti-mouse ICOS BV510 (C398.4A)	BioLegend	Cat# 313525; RRID:AB_2562642
Anti-mouse PD-1 PE-Cy7 (RMP1-30)	BioLegend	Cat# 109110; RRID:AB_572017
Anti-mouse PD-1 BUV805 (29F.1A12)	BD Biosciences	Cat# 568608
Anti-mouse CTLA-4 PE-Dazzle594 (UC10-4B9)	BioLegend	Cat# 106318; RRID:AB_2564496
Anti-mouse CTLA-4 APC (UC10-4F10-11)	BD Biosciences	Cat# 564331; RRID:AB_2738751
Anti-mouse CTLA-4 PE (UC10-4F10-11)	BD Biosciences	Cat# 553720; RRID:AB_395005
Biotin anti-Allophycocyanin (APC003)	BioLegend	Cat# 408004; RRID:AB_345360
Anti-mouse IL-10 BV421 (JES5-16E3)	BioLegend	Cat# 505022; RRID:AB_2563240
Anti-mouse CD16/32 (93)	BioLegend	Cat# 101320; RRID:AB_1574975
Anti-mouse CD5 AF488 (53–7.3)	BioLegend	Cat# 100612; RRID:AB_493165
Anti-mouse CD19 AF488 (6D5)	BioLegend	Cat# 115521; RRID:AB_389307
Anti-mouse GR-1 AF488 (RB6-8C5)	BioLegend	Cat# 108417; RRID:AB_389309
Anti-mouse NK1.1 AF488 (PK136)	BioLegend	Cat# 108718; RRID:AB_493183
Anti-mouse CD11c PE-Cy7 (N418)	BioLegend	Cat# 117318; RRID:AB_493568
Anti-mouse I-Ab eFluor450 (M5/114.15.12)	eBioscience (ThermoFisher)	Cat# 48-5321-82; RRID:AB_1272204
Anti-mouse I-Ak BV421 (10–3.6)	BD Biosciences	Cat# 743471; RRID:AB_2741527
Anti-mouse CD11b Percp-Cy5.5 (M1/70)	BD Biosciences	Cat# 550993; RRID:AB_394002
Anti-mouse 33D1 APC (33D1)	BioLegend	Cat# 124914; RRID:AB_1227625
Anti-mouse CD8a PE (53–6.7)	BioLegend	Cat# 100708; RRID:AB_312747
Anti-mouse CD8a BV510 (53–6.7)	BioLegend	Cat# 100751; RRID:AB_2561389
Anti-mouse CD8a AF488 (53–6.7)	BioLegend	Cat# 100723; RRID:AB_389304
Anti-mouse CD8a Bv711 (53–6.7)	BioLegend	Cat# 100747; RRID:AB_11219594
Anti-mouse Sirpa BUV805 (P84)	BD Biosciences	Cat# 741997; RRID:AB_2871296
Anti-mouse XCR1 BV510 (ZET)	BioLegend	Cat# 148218; RRID:AB_2565231
Anti-mouse CD103 APC (2E7)	BioLegend	Cat# 121414; RRID:AB_1227502
Anti-mouse F4/80 BV711 (BM8)	BioLegend	Cat# 123147; RRID:AB_2564588
Anti-mouse CD80 BV605 (16-10A1)	BioLegend	Cat# 104729; RRID:AB_11126141
Anti-mouse CD86 BV650 (GL-1)	BioLegend	Cat# 105036; RRID:AB_2686973
Anti-mouse CD40 PE-Dazzle594 (3/23)	BioLegend	Cat# 124630; RRID:AB_2572185
Anti-mouse CD45.2 BUV395 (104)	BD Biosciences	Cat# 564616; RRID:AB_2738867
Anti-mouse CD45.1 AF700 (A20)	BioLegend	Cat# 110723; RRID:AB_493732
Anti-mouse IL-10R PE (1B1.3a)	BioLegend	Cat# 112705; RRID:AB_313518
Anti-mouse CD73 PE-Cy7 (TY/11.8)	BioLegend	Cat# 127224; RRID:AB_2716103
Anti-mouse FR4 PE-Dazzle594 (12A5)	BioLegend	Cat# 125015; RRID:AB_2721703
TotalSeq-C0301 anti-mouse Hashtag 1	BioLegend	Cat# 155861; RRID:AB_2800693
TotalSeq-C0302 anti-mouse Hashtag 2	BioLegend	Cat# 155863; RRID:AB_2800694
TotalSeq-C0303 anti-mouse Hashtag 3	BioLegend	Cat# 155865; RRID:AB_2800695
Anti-mouse CTLA-4 (UC10-4F10-11)	Bio X Cell	Cat# BP0032
Anti-glutathione S-transferase (PIP)	Bio X Cell	Cat# BE0260
Bacterial and virus strains
XL-1 Blue E.coli	Daniel J. Campbell Lab	N/A
Chemicals, peptides, and recombinant proteins
Liberase TM	Roche	Cat# 05401127001
DNase I	Sigma-Aldrich	Cat# D4513
Collagenase Type V	Sigma-Aldrich	Cat# C9263
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester	Sigma-Aldrich	Cat# 21888
Enzyme-free cell dissociation buffer	Sigma-Aldrich	Cat# C5789
Bafilomycin A1	Sigma-Aldrich	Cat# SML1661
ACK lysis buffer	ThermoFisher	Cat# A1049201
LPS	Sigma-Aldrich	Cat# L4005
Fixable Viability Dye eFluor780	ThermoFisher	Cat# 65-0865-18
Streptavidin-APC	BioLegend	Cat# 405207
PD-1 Ig	Bio-Techne	Cat# 1021-PD
Critical commercial assays
CD11c MicroBeads, mouse	Miltenyi Biotec	Cat# 130-125-835
CD4 MicroBeads, mouse	Miltenyi Biotec	Cat# 130-117-043
CD4+ T cell Isolation Kit, mouse	Miltenyi Biotec	Cat# 130-104-454
Dynabeads mouse T cell activator	ThermoFisher	Cat# 11456D
Cell Stimulation Cocktail (plus protein transport inhibitors)	ThermoFisher	Cat# 00-4975-03
eBioscience Foxp3/Transcription Factor Staining Buffer Set	ThermoFisher	Cat# 00-5523-00
SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing	Takara Bio	Cat# 634894
NexteraXT DNA Library Preparation Kit	Illumina	Cat# FC-131-1024
Library Construction Kit, 16 rxns	10x Genomics	Cat# 1000190
Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns	10x Genomics	Cat# 1000254
Chromium Next GEM Single Cell 5′ Kit v2, 4 rxns	10x Genomics	Cat# 1000265
Chromium Next GEM Chip K Single Cell Kit, 16 rxns	10x Genomics	Cat# 1000287
5′ Feature Barcode Kit, 16 rxns	10x Genomics	Cat# 1000256
Dual Index Kit TN Set A, 96 rxn	10x Genomics	Cat# 1000250
Dual Index Kit TT Set A 96 rxns	10x Genomics	Cat# 1000215
NextSeq 2000 P3 Reagents (100 Cycles)	Illumina	Cat# 20040559
R-PE Lightning-Link Conjugation Kit	AbCam	Cat# AB102918
Deposited data
Bulk and single-cell RNA-sequencing data	–	Gene Expression Omnibus (GEO) series GEO: GSE249482
Code used for bulk and single-cell RNA-sequencing analyses		http://doi.org/10.5281/zenodo.13315646
Experimental models: Cell lines
Platinum-E	Shivani Srivastava Lab, Fred Hutchinson Cancer Center	N/A
NIH/3T3	Daniel J. Campbell Lab	N/A
Experimental models: Organisms/strains
C57BL/6J	Jackson Laboratory	Cat# 000664
B6.SJL-PtprcaPepcb/BoyJ	Jackson Laboratory	Cat# 002014
B6.129S2-Il10rbtm1Agt/J	Jackson Laboratory	Cat# 005027
C57BL/6-Foxp3tm1Flv/J	Jackson Laboratory	Cat# 008374
NOD/ShiLtJ	Jackson Laboratory	Cat# 001976
BALB/cJ	Jackson Laboratory	Cat# 000651
CD80-mCherry.Rag2−/−	Lucy S.K. Walker and David M. Sansom Labs, University College London	N/A
Recombinant DNA
pCD86-EGFP	Addgene	Cat# 133858
modified MSCV2.2	Daniel J. Campbell Lab	N/A
Software and algorithms
FlowJo v10.8 and v10.9. Software	BD Life Sciences	N/A
R 4.0.3	CRAN	N/A
limma_3.49.4	https://bioconductor.org/packages/release/bioc/html/limma.html	N/A
ROAST	https://bioconductor.org/packages/release/bioc/html/limma.html	N/A
STAR v.2.4.2a	https://github.com/alexdobin/STAR	N/A
HTSeq 2.0.2	https://pypi.org/project/HTSeq/	N/A
Picard tools 1.134	https://github.com/broadinstitute/picard	N/A
Seurat_v3	https://satijalab.org/seurat/	N/A
CellRanger	https://www.10xgenomics.com/support/software/cell-ranger/getting-started/cr-what-is-cell-ranger#	N/A
ComplexHeatmap	https://bioconductor.org/packages/release/bioc/html/ComplexHeatmap.html	N/A
EdgeR	https://bioconductor.org/packages/release/bioc/html/edgeR.html	N/A