Extended Data Fig. 9. Potential fitness effects of acclimating D. melanogaster and D. sechellia to 16:8 h LD.
a, Two replicates (top and bottom) of the cumulative survival probability for D. melanogaster (DmelCS and DmelOR) maintained at 12:12 h LD or 16:8 h LD. No significant differences were observed between strains of the same species by photoperiod. b, Two replicates (top and bottom) of the cumulative survival probability for D. sechellia (Dsec07 and Dsec28) maintained at 12:12 h LD or 16:8 h LD. No significant differences were observed between strains of the same species by photoperiod. For a-b, asterisks indicate significant differences between photoperiod treatments of the same strain: * = P < 0.05, ** = P < 0.01 and *** = P < 0.001 (log-rank test). The top replicate in both panels is the same data presented in Fig. 5f (where it is pooled by species). c, Percent of copulating pairs observed for D. melanogaster (blue) and D. sechellia (orange) after 3 d for flies acclimated to 12:12 h LD (left) compared to 16:8 h LD (right). Error bars, representing 95% confidence intervals, were calculated using 1000 bootstraps. * = P < 0.05 and ** = P < 0.01 (Wilcoxon tests with Bonferroni correction for multiple comparisons).