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. 2024 Oct 16;635(8040):951–959. doi: 10.1038/s41586-024-08056-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 4 — a, Mean normalized activity of the indicated Dmel genotypes under 12:12 h LD (left) and 16:8 h LD (right). n: 12:12 h LD, DmelPdf-Gal4/+ (31), DsecPdf-Gal4/+ (29), UAS-Pdf^RNAi/+ (47), DmelPdf-Gal4/UAS-Pdf^RNAi (34), DsecPdf-Gal4/UAS-Pdf^RNAi (30); 16:8 h LD, DmelPdf-Gal4/+ (34), DsecPdf-Gal4/+ (32), UAS-Pdf^RNAi/+ (16), DmelPdf-Gal4/UAS-Pdf^RNAi (31), DsecPdf-Gal4/UAS-Pdf^RNAi (24). b,c, Evening peak time for the flies shown in a under 12:12 h LD (b) and 16:8 h LD (c). d, Mean normalized predawn activity for the genotypes shown in a under 12:12 h LD. e, Mean normalized activity of the Pdf mutant and Pdf-rescue genotypes under 12:12 h LD (top) and 16:8 h LD (bottom) (combined data from two independent lines). n: 12:12 h LD, Pdf⁰¹ (30); DmelPdf,Pdf⁰¹ (89); DsecPdf,Pdf⁰¹ (86); 16:8 h LD, Pdf⁰¹ (16), DmelPdf,Pdf⁰¹ (59); DsecPdf,Pdf⁰¹ (79). f,g, Evening peak time for flies under 12:12 h LD (f) and 16:8 h LD (g) shown in e. n: Pdf⁰¹ (16); DmelPdf,Pdf⁰¹ no. 1 (29); DmelPdf,Pdf⁰¹ no. 2 (30); DsecPdf,Pdf⁰¹ no. 1 (38); DsecPdf,Pdf⁰¹ no. 2 (41). h, Mean normalized predawn activity for flies depicted in e under 12:12 h LD. n: Pdf⁰¹ (30); DmelPdf¹,Pdf⁰¹ (40); DmelPdf¹,Pdf⁰¹ (49); DsecPdf¹,Pdf⁰¹ (44); DsecPdf²,Pdf⁰¹ (42). b–d,f–h, *P < 0.05, ***P < 0.001 (Wilcoxon tests with Bonferroni correction). f–h, Results for independent species-specific rescue strains and pooled rescue strains are shown in box plots to the left and right of the dashed line, respectively; letters A–C indicate significant differences, P < 0.05 (Wilcoxon tests with Bonferroni correction).

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