Fig. 4.

Increased numbers of CD27⁺IgD⁺ NSw MBCs with elevated local IgM, PD-L1, and IL-10 expression in the livers of HCC/CaMIN mice.

(A) Gating strategy to identify and characterize the phenotype of MBCs in the liver. (B–E) Frequencies of (B) CD27^-IgD^- DN, (C) CD27⁺IgD⁺ NSw, (D) CD27⁺IgD^- Sw, and (E) CD27^-IgD⁺ MN MBCs. (F, G) Frequencies of IgM⁺-, PD-L1⁺- and IL-10⁺-expressing (F) CD27⁺IgD⁺ NSw and (G) CD27⁺IgD^- Sw MBCs. The data were analyzed using the unpaired Student’s t test. The data were shown as the mean ± SEM, n = 6. ∗p <0.05, ∗∗p <0.01, ∗∗∗∗p <0.0001. Fig. S4 shows the MASLD and HCC/NRAS^G12V/p19^Arf-/- models. DN, double-negative; FSC-A, forward scatter area; FSC-H, forward scatter height; HCC, hepatocellular carcinoma; MBCs, memory B cells; NSw, non-switched; PD-L1, programmed death-ligand 1; SSC-H, side scatter height; Sw, switched.