Fig. 5.

There was a strong increase in the frequencies of PD-L1⁺-, IL-10⁺-, and IgM⁺IgD⁺-expressing CD19⁺B220⁺CD138⁺ PBs in the livers of HCC/CaMIN mice.

(A) Gating strategy. (B) Frequencies of CD19⁺B220⁺CD138⁺ PBs. (C, D) Frequencies of (C) PD-L1⁺- and (D) IL-10⁺-expressing CD19⁺B220⁺CD138⁺ PBs. (E–H) Frequencies of (E) IgM⁺IgD^--, (F) IgM⁺IgD⁺-, (G), IgA^-IgD⁺-, and (H) IgA⁺IgD^--expressing CD19⁺B220⁺CD138⁺ PBs. The data were analyzed using the unpaired Student’s t test. The data are shown as the mean ± SEM, n = 6. ∗p <0.05, ∗∗p <0.01, ∗∗∗p <0.001, ∗∗∗∗p <0.0001. Fig. S5 shows the MASLD and HCC/NRAS^G12V/p19^Arf-/- models. FSC-A, forward scatter area; FSC-H, forward scatter height; HCC, hepatocellular carcinoma; PBs, plasmablasts; PD-L1, programmed death-ligand 1; SSC-H, side scatter height.