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Deletion of the SSY1 or the AGP1 gene affects the utilization of several amino acids in cells lacking the general amino acid permease (Gap1p). Cells were spread on minimal medium with the indicated amino acid at the final concentration of 1 mM (A) or 10 mM (B) as the sole nitrogen source. The strains were 23344c (ura3), 32501b (gap1Δ ura3), 32501d (gap1Δ ssy1Δ ura3), 30633c (gap1Δ agp1Δ ura3), and 32502b (gap1Δ ssy1Δ agp1Δ ura3).
