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Deletion of the SSY1 or AGP1 gene alters incorporation of amino acids. The time course of 14C-labeled leucine, isoleucine, phenylalanine, and tyrosine (initial concentration, 0.1 mM) accumulation measured in cells growing on minimal medium with urea as the sole nitrogen source is shown. The strains were 30629c (gap1Δ ura3) (■), 30633c (gap1Δ agp1Δ ura3) (□) and 32501d (gap1Δ ssy1Δ ura3) (○).
