TABLE 2.

The Ssy1 protein is required for transcriptional induction of the AGP1 gene in response to multiple amino acids^a

Line no.	Nitrogen sources	AGP1-lacZ β-galactosidase activity (nmol · min−1 · mg of protein−1)
		Wild type	ssy1Δ
1	Urea	≤2	≤2
2	Urea + Leu	713	≤2
3	Urea + IIe	1,069	≤2
4	Urea + Phe	943	≤2
5	Urea + Tyr	903	≤2
6	Urea + Trp	1,246	≤2
7	Urea + Thr	871	≤2
8	Urea + Met	825	≤2
9	Urea + Val	421	≤2
10	Urea + citrulline	294	≤2
11	Urea + Cys	241	≤2
12	Urea + Ala	198	≤2
13	Urea + Ser	177	≤2
14	Urea + Lys	84	≤2
15	Urea + His	75	≤2
16	Urea + Glt	54	≤2
17	Urea + Gln	49	≤2
18	Urea + Gly	46	≤2
19	Urea + Asp	33	≤2
20	Urea + Asn	27	≤2
21	Urea + GABA	7	≤2
22	Urea + Arg	5	≤2
23	Urea + ornithine	4	≤2
24	Urea + Pro	≤2	≤2
25	Urea + allantoin	≤2	≤2
26	Urea + adenine	≤2	≤2
27	Urea + cytosine	≤2	≤2
28	Urea + allantoate	≤2	≤2

^aStrains 23344c (ura3) and 30501c (ssy1Δ ura3) transformed with the CEN-based YCpAGP1-lacZ plasmid were grown on a minimal medium with the indicated compounds as the sole nitrogen source(s), each added at 5 mM final concentration. The reported β-galactosidase activities are means of two to three independent experiments. Variations were less than 20%.