Fig. 3. Evolutionary relationships of the Alpha variant in Cambodia.

a Maximum likelihood phylogeny of SARS-CoV-2 viruses inferred from Nextstrain tree in Fig. 2. Light blue tip circles represent subsampled global Alpha sequences. Deep pink tip circles denote Cambodian sequences obtained from SARS-CoV-2 positive patients. Cambodian Alpha sequences that have acquired the spike E484K mutation are highlighted in the shaded purple area. Amino acid mutations are mapped at the major nodes. b Proportion of sequences representing different groups of Alpha and Delta variants within Cambodia. c Vaccination coverage over time among the Cambodian population (with one dose represented by the red line and two doses by the green line). The purple line displays the proportion of sequenced Alpha viruses with the E484K mutation.