Table 2.

Molecular loci, PCR primers and programmes used in this study.

Loci	PCR primers	Sequence (5’–3’)	PCR cycles	References
ITS	ITS5	GGA AGT AAA AGT CGT AAC AAG G	95 °C 5 min; (95 °C 30 s, 55 °C 30 s, 72 °C 1 min) × 35 cycles; 72 °C 10 min	White et al. (1990)
	ITS4	TCC TCC GCT TAT TGA TAT GC
LSU	LR0R	GTA CCC GCT GAA CTT AAG C	95 °C 5 min; (95 °C 50 s, 47 °C 30 s, 72 °C 1.5 min) × 35 cycles; 72 °C 10 min	Vilgalys and Hester (1990)
	LR7	TAC TAC CAC CAA GAT CT
TEF	EF1-728F	CAT CGA GAA GTT CGA GAA GG	95 °C 5 min; (95 °C 30 s, 55 °C 60 s, 72 °C 1 min) × 30 cycles; 72 °C 10 min	Carbone and Kohn (1999); O’Donnell et al. (1998)
	EF2	GGA RGT ACC AGT SAT CAT GTT
RPB1	RPB1-Af	GAR TGY CCD GGD CAY TTY GG	95 °C 3 min; (94 °C 40 s, 60 °C 40 s, 72 °C 2 min) × 9 (94 °C 45 s, 55 °C 1.5 min, 72 °C 2 min) × 37 cycles; 72 °C 10 min	Stiller and Hall (1997)
	RPB1-Cr	CCN GCD ATN TCR TTR TCC ATR TA
ACT	ACT-512F	ATG TGC AAG GCC GGT TTC GC	95 °C 3 min; (95 °C 1 min, 55 °C 1 min, 72 °C 1 min) × 30 cycles; 72 °C 10 min	Voigt and Wostemeyer (2000)
	ACT-783R	TAC GAG TCC TTC TGG CCC AT