Table 1. Primers Used in the Design of the Recombinant Constructs.

constructs	primer
rHIFα (α) Construct Proteins
N-α	forward: 5′ ccatgggccatcatcatca 3′
	reverse: 5′ taactagtaatacgggtttcttcgctatcc 3′
CRD-α	forward: 5′ atcctaggaaacaggatctgcagacac 3′
	reverse: 5′ taactagtaatacgggtttcttcgctatcc 3′
α-C	forward: 5′ atcctaggaaacaggatctgcagacac 3′
	reverse: 5′ ggccggatccttaactagtatagat 3′
rHIFγ(C387S) (γ) Construct Proteins
N-γ	forward: 5′ taccatgggccatcatcatc 3′
	reverse: 5′ taactagtcaccatcagttcttgg 3′
CRD-γ	forward: 5′ tacctaggaaaaacattctggg 3′
	reverse: 5′ taactagtcaccatcagttcttgg 3′
γ-C	forward: 5′ tacctaggaaaaacattctggg 3′
	reverse: 5′ taactagtctgcagaataatg 3′