Table 2. Average Protein Recoveries and Purity Levels of the rHIF Constructs.
protein construct | molecular weight (kDa) | theoretical pI | estimated protein purity (%) | purification yield (g kg–1) | references |
---|---|---|---|---|---|
N-α-C | 70.50 | 7.94 | 88 | 39 | (17) |
N-α | 58.10 | 6.88 | 83 | 65 | (this work) |
α central rod | 39.58 | 6.03 | 78 | 44 | (this work) |
α-C | 51.61 | 6.09 | 83 | 37 | (this work) |
N-γ-C | 66.67 | 5.52 | 91 | 45 | (17) |
N-γ | 54.56 | 4.90 | 88 | 86 | (this work) |
γ central rod | 40.27 | 4.57 | 78 | 107 | (this work) |
γ-C | 52.00 | 4.78 | 86 | 59 | (this work) |