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. 2024 Nov 14;9(47):47023–47030. doi: 10.1021/acsomega.4c06950

Table 2. Average Protein Recoveries and Purity Levels of the rHIF Constructs.

protein construct molecular weight (kDa) theoretical pI estimated protein purity (%) purification yield (g kg–1) references
N-α-C 70.50 7.94 88 39 (17)
N-α 58.10 6.88 83 65 (this work)
α central rod 39.58 6.03 78 44 (this work)
α-C 51.61 6.09 83 37 (this work)
N-γ-C 66.67 5.52 91 45 (17)
N-γ 54.56 4.90 88 86 (this work)
γ central rod 40.27 4.57 78 107 (this work)
γ-C 52.00 4.78 86 59 (this work)